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enzyme can bind but a single divalent cation within the Lively site. For the binuclear metallic Middle, the activation on the amide bond as well as activation of the solvent water is often dispersed individually between The 2 steel ions. Having said that, from the mononuclear metal Middle, equally features needs to be borne by only one divalent cation. These effects highlight the numerous variety for that evolution of functionality throughout the amidohydrolase superfamily.

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with a battery of substrate analogs and Energetic site mutants employing a host of metal-substituted sorts of the enzyme. We conclude from these scientific studies that NagA from E. coli

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